Kigamicin D is produced by the strain of Amycolatopsis sp. ML-630-mFl. It showed activity against gram-positive bacteria including methicillin-resistant Staphylococcus aureus (MRSA) with MIC of 0.025-0.78 μg/mL. It also shows effect against L-1210 LB32T and other genera tumor cells with IC50 of 1 μg/mL.